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020 _a9781493973668
024 7 _a10.1007/978-1-4939-7366-8
_2doi
040 _aBIBLIOTECA UNIBE
_cBIBLIOTECA UNIBE
041 _aspa
050 4 _aQD431-431.7
072 7 _aPSBC
_2bicssc
072 7 _aSCI007000
_2bisacsh
082 0 4 _a572.6
_223
245 1 0 _aProtein Engineering
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Uwe T. Bornscheuer, Matthias Höhne.
264 1 _aNew York :
_bSpringer New York :
_bImprint: Humana Press,
_c2018.
300 _aXI, 352 p. 82 illus., 63 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1064-3745 ;
_v1685
505 0 _aProtein Engineering - Past, Present, and Future -- (Semi)rational Protein Design -- Computational Analysis of Protein Tunnels and Channels -- YASARA - A Tool to Obtain Structural Guidance in Biocatalytic Investigations -- A Computational Library Design Protocol for Rapid Improvement of Protein Stability – FRESCO -- Directed Evolution of Proteins Based on Mutational Scanning -- A Brief Guide to the High-throughput Expression of Directed Evolution Libraries -- Library Growth and Protein Expression: Optimal and Reproducible Microtiter Plate Expression of Recombinant Enzymes in E. coli using MTP Shakers -- Normalized Screening of Protein Engineering Libraries by Split-GFP Crude Cell Extract Quantification -- Functional Analysis of Membrane Proteins Produced by Cell-Free Translation -- Practical Considerations Regarding the Choice of the Best High-throughput Assay -- High-throughput Screening Assays for Lipolytic Enzymes -- Continuous High-throughput Colorimetric Assays for α-transaminases -- Colorimetric High-troughput Screening Assays for the Directed Evolution of Fungal Laccases -- Directed Co-evolution of Two Cellulosic Enzymes in Escherichia coli Based on their Synergistic Reactions -- Program-guided Design of High-throughput Enzyme Screening Experiments and Automated Data Analysis/Evaluation -- Solid Phase Agar Plate Assay for Screening Amine Transaminases -- Ultrahigh-throughput Screening of Single-cell Lysates for Directed Evolution and Functional Metagenomics -- Isolation of pH-sensitive Antibody Fragments by Fluorescence-activated Cell Sorting and Yeast Surface Display -- Library Generation and Auxotrophic Selection Assays in Escherichia coli and Thermus thermophiles. .
506 0 _aOpen Access
520 _aThis volume details basic and advanced protocols for both stages of protein engineering: the library design phase and the identification of improved variants by screening and selection. Chapters focus on enzyme engineering using rational and semi-rational approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Protein Engineering: Methods and Protocols aims to aid scientists in the planning and performance of their experiments. .
650 0 _aLife sciences.
_9153057
650 0 _aProteins.
_9153058
650 1 4 _aLife Sciences.
_9153059
650 2 4 _aProtein Science.
_9153060
700 1 _aBornscheuer, Uwe T.
_eeditor.
_9153061
700 1 _aHöhne, Matthias.
_eeditor.
_9153062
710 2 _aSpringerLink (Online service)
_953287
773 0 _tSpringer eBooks
776 0 8 _iPrinted edition:
_z9781493973644
830 0 _aMethods in Molecular Biology,
_x1064-3745 ;
_v1685
_9153063
856 4 0 _uhttp://dx.doi.org/10.1007/978-1-4939-7366-8
856 4 0 _uhttp://www.springerprotocols.com/doiresolver?genre=book&pid=10.1007/978-1-4939-7366-8
942 _2ddc
_cBK