000 | 04184nam a22004935i 4500 | ||
---|---|---|---|
999 |
_c48281 _d48281 |
||
001 | 978-1-4939-7366-8 | ||
003 | DE-He213 | ||
005 | 20221003091138.0 | ||
007 | cr nn 008mamaa | ||
008 | 171030s2018 xxu| s |||| 0|eng d | ||
020 | _a9781493973668 | ||
024 | 7 |
_a10.1007/978-1-4939-7366-8 _2doi |
|
040 |
_aBIBLIOTECA UNIBE _cBIBLIOTECA UNIBE |
||
041 | _aspa | ||
050 | 4 | _aQD431-431.7 | |
072 | 7 |
_aPSBC _2bicssc |
|
072 | 7 |
_aSCI007000 _2bisacsh |
|
082 | 0 | 4 |
_a572.6 _223 |
245 | 1 | 0 |
_aProtein Engineering _h[electronic resource] : _bMethods and Protocols / _cedited by Uwe T. Bornscheuer, Matthias Höhne. |
264 | 1 |
_aNew York : _bSpringer New York : _bImprint: Humana Press, _c2018. |
|
300 |
_aXI, 352 p. 82 illus., 63 illus. in color. _bonline resource. |
||
336 |
_atext _btxt _2rdacontent |
||
337 |
_acomputer _bc _2rdamedia |
||
338 |
_aonline resource _bcr _2rdacarrier |
||
347 |
_atext file _bPDF _2rda |
||
490 | 1 |
_aMethods in Molecular Biology, _x1064-3745 ; _v1685 |
|
505 | 0 | _aProtein Engineering - Past, Present, and Future -- (Semi)rational Protein Design -- Computational Analysis of Protein Tunnels and Channels -- YASARA - A Tool to Obtain Structural Guidance in Biocatalytic Investigations -- A Computational Library Design Protocol for Rapid Improvement of Protein Stability – FRESCO -- Directed Evolution of Proteins Based on Mutational Scanning -- A Brief Guide to the High-throughput Expression of Directed Evolution Libraries -- Library Growth and Protein Expression: Optimal and Reproducible Microtiter Plate Expression of Recombinant Enzymes in E. coli using MTP Shakers -- Normalized Screening of Protein Engineering Libraries by Split-GFP Crude Cell Extract Quantification -- Functional Analysis of Membrane Proteins Produced by Cell-Free Translation -- Practical Considerations Regarding the Choice of the Best High-throughput Assay -- High-throughput Screening Assays for Lipolytic Enzymes -- Continuous High-throughput Colorimetric Assays for α-transaminases -- Colorimetric High-troughput Screening Assays for the Directed Evolution of Fungal Laccases -- Directed Co-evolution of Two Cellulosic Enzymes in Escherichia coli Based on their Synergistic Reactions -- Program-guided Design of High-throughput Enzyme Screening Experiments and Automated Data Analysis/Evaluation -- Solid Phase Agar Plate Assay for Screening Amine Transaminases -- Ultrahigh-throughput Screening of Single-cell Lysates for Directed Evolution and Functional Metagenomics -- Isolation of pH-sensitive Antibody Fragments by Fluorescence-activated Cell Sorting and Yeast Surface Display -- Library Generation and Auxotrophic Selection Assays in Escherichia coli and Thermus thermophiles. . | |
506 | 0 | _aOpen Access | |
520 | _aThis volume details basic and advanced protocols for both stages of protein engineering: the library design phase and the identification of improved variants by screening and selection. Chapters focus on enzyme engineering using rational and semi-rational approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Protein Engineering: Methods and Protocols aims to aid scientists in the planning and performance of their experiments. . | ||
650 | 0 |
_aLife sciences. _9153057 |
|
650 | 0 |
_aProteins. _9153058 |
|
650 | 1 | 4 |
_aLife Sciences. _9153059 |
650 | 2 | 4 |
_aProtein Science. _9153060 |
700 | 1 |
_aBornscheuer, Uwe T. _eeditor. _9153061 |
|
700 | 1 |
_aHöhne, Matthias. _eeditor. _9153062 |
|
710 | 2 |
_aSpringerLink (Online service) _953287 |
|
773 | 0 | _tSpringer eBooks | |
776 | 0 | 8 |
_iPrinted edition: _z9781493973644 |
830 | 0 |
_aMethods in Molecular Biology, _x1064-3745 ; _v1685 _9153063 |
|
856 | 4 | 0 | _uhttp://dx.doi.org/10.1007/978-1-4939-7366-8 |
856 | 4 | 0 | _uhttp://www.springerprotocols.com/doiresolver?genre=book&pid=10.1007/978-1-4939-7366-8 |
942 |
_2ddc _cBK |